Second- and third-generation biodiesel production with immobilised recombinant Rhizopus oryzae lipase: Influence of the support, substrate acidity and bioprocess scale-up

• Rhizopus oryzae lipase proved an excellent biocatalyst for biodiesel production. Hydrocarbon chains on the support increased operational stability. Low oil acidity negatively influenced Acyl-acceptor concentration and had a synergistic effect. Biodiesel production from waste cooking was successfully scaled up. immobilised onto differently functionalised polymethacrylate (Purolite®) magnetite superparamagnetic supports assessed as catalyst with pomace oil. The presence of surface hydrocarbon stability biocatalysts supported Purolite® particles up to 9 2 times, respectively. By contrast, functional groups no effect initial transesterification rate, which twice higher Purolite®. Also, functionalising epoxide octadecyl led highest volumetric productivity. This other substrates including makauba, jatropha, oil, microbial similar reaction rates. However, simply raising substrate 0.5 2% 15 times. A between acyl-acceptor observed. 50 mL.